2017;7(05):161–5. 2012;1(9):239–42. In this study, Acetyl Salicylic Acid (ASA) was used as a reference standard. Cold and sore throats. Table 1. 23. The villagers were mainly willing to share their information of herbal medication. To determine the cytotoxicity of plant extract, brine shrimp lethality assay is an important parameter by observing the capability of extract to kill a laboratory cultured larvae (nauplii). Afr J Biotechnol. Lamina green to yellowish green, deltoid, pentagonal-ovate, or broadly ovate-lanceolate, shorter than stipe, 4-18 × 4-12 cm, tripinnate or pinnate-bipinnatifid, herbaceous, thin, glabrous abaxially, with sparse, short hairs adaxially; rachis and costae grooved adaxially, margins of grooves narrowly winged on distal rachis and costae. Correspondence to Khatun M, Rahman M, Haque T, Rahman MM, Akter M, Akter S, Jhumur A. Cytotoxicity potentials of eleven Bangladeshi medicinal plants. Aliquots of blood was then transferred to the previously weighed sterile Eppendorf tubes and was incubated at 37 °C for 45 min to form clot. Cheilanthes tenuifolia (Burm. Cheilanthes - small evergreen ferns: lipferns; in some classifications placed in … Fil. In our study, methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE), (Table 2). Indica, 237. References. Dhar KS, Wahed TB, Hasan AN, Wahed SB. The other compounds like carbohydrates, glycosides, tannins, and alkaloids remain as medium intensity in the plant. Evaluation of antioxidant activity of two important memory enhancing medicinal plants Baccopa monnieri and Centella asiatica. Results of membrane stabilizing potential (hypotonic solution induced and heat induced hemolysis) of different extracts is summarized in (Table 3). (Sinopteridaceae) Common name: Narrow-leaved lip Fern, Resam Lumut. Manage cookies/Do not sell my data we use in the preference centre. 2014;4(1):359–67. Cheilanthes austrotenuifolia: is known as the Rock Fern. Cheilanthes tenuifolia (N. L. Burman) Swartz, Syn. In present study, after performing silica column chromatographic technique and TLC technique, several possible compounds were isolated through elution from n-Hexane fraction. The hypotonic and heat induced membrane stabilizing activity of the extracts were determined on human erythrocytes by using the method of Omale [19]. Google ScholarÂ. Satpura Hills Hoshangabad, Betul, Chhindwara, Damoh, Sidhi, Khandwa, Mandla, Amarkantak (Anuppur) and Panna General tonic 22. Although this forest is surrounded by rapid socio-economic development, it is ironically a substantial pharmacopoeia for the Temuans. Cheilanthes tenuifolia Swartz. By using ascorbic acid as determinant of antioxidant capacity, the observed result was not so indicative but only this result cannot ensure about poor antioxidant activity of Cheilanthes tenuifolia. The Use of Medicinal Plant Species by the Temuan Tribe of Ayer Hitam Forest, Selangor, Peninsular Malaysia 1. In another beaker 90 mL of methanol was mixed with 10 mL of water. Cookies policy. Difference obtained in weight taken before and after clot lysis was expressed as percentage of clot lysis [18]. TLC is the most applied method of isolation of bioactive compounds from plants [23]. Cheilanthes austrotenuifolia is a species of Australian rock fern from the family Pteridaceae.Once a common understory plant along streams in moist areas of Southern Australia, Cheilianthes austrotenuifolia is still quite widespread in remnant bush-land, such as areas of the Adelaide Hills, although it has been affected significantly by land clearance and competition from invasive species. On the other hand sterile distilled water, a negative control exhibited a negligible percentage of lysis of clot 8.511%, ethyl acetate extract 31.594%, N-hexane fraction 12.100%, methanol fraction 17.006% and chloroform fraction 41.261% showed lysis of clot respectively. In the shelter of a large lump of granite this elegant fern was growing. 2015;7(4):960–2. Then cotton filtration process was performed to get supernatant and rotary evaporator was used to dry the extract. Presence of tannin was not so much prominent in Cheilanthes tenuifolia plant. Cite this article. All extracts were undergone for thrombolytic activity and compared against standard streptokinase. The sample extract (0.2 mL) was diluted with methanol and 2 mL of DPPH solution (0.5 mM) was added. All the authors reviewed the manuscript and provide consent for publication. Caius (1935) is supposed to be the first man who has described medicinal uses of some ferns of India. The capacity of chloroform and ethyl acetate fractions are also noteworthy (98.24 μg/ml and 77.99 μg/ml) and N-hexane showed minimal (334.76 μg/ml) activity to DPPH as increased IC50 value decreases inhibitory activity. The extract was further partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method. Then % inhibitions were plotted against log concentration and from the graph IC50 was calculated. Then slowly the mixture was added to the extract to make an aqueous extract solution. Sample extracts (0.3 mL) was combined with 3 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate). Preliminary results of a study on the use of medicinal plant species by the indigenous people from the Temuan tribe at Ayer Hitam Forest are presented. Sources of data Cheilanthes tenuifolia (inventories, collectors / déterminateurs, organizations, data transmission) In continuing your browsing of this site, you accept the use of cookies to offer you suitable content and services and realize visits statistics. Medicinal ferns of north eastern India with special reference to Arunachal Pradesh. 2008;7(17):3129–33. As the erythrocyte membrane is similar to lysosomal membrane and drug effects on erythrocyte stabilization could likely be to the stabilization of lysosomal membrane [27]. In prehistoric time, the rhizome juice of ferns was used to cure GI disorders as well as peptic ulcer and the rhizome paste of ferns for healing of cuts and wounds. Indian J Tradit Know. Free radicals generated by metabolic reactions and/or exogenous chemicals are responsible for oxidation of bio-molecules like nucleic acids, lipids, proteins, etc. PubMed Google Scholar. Gallic acid (GA) was taken as standard that provided a standard curve (y = 0.0043x – 0.0723, R2 = 0.9803) to determine the total phenol content in different fractions. Privacy Wagenen BCV, Larsen R, Cardellina JH, Ran D, Lidert ZC, Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosaruetzleri. It’s usually found in open, moist shady and rocky mountain area, but sometimes also grown in moist shady habitat [7]. 2017;8(3):1476–84. Click below on a thumbnail map or name for species profiles. Sci World J. the optical density (OD) of the supernatant was measured at 540 nm. Commercially available lyophilized Streptokinase (1,500,000 I.U) was collected and 5 ml sterile distilled water was added to it and mixed properly. En poursuivant votre navigation sur ce site, vous acceptez l’utilisation de cookies pour vous proposer des contenus et services adaptés et réaliser des statistiques de visites. Our current study suggests that Cheilanthes tenuifolia possess very poor total antioxidant capacity. In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC50 = 34.493 μg/ml) compared to other plant extracts. Omale J, Okafor PN. After performing silica column chromatography, TLC and PTLC technique, several possible compounds were isolated through elution from n-Hexane fraction and sample studied for 1H-NMR spectroscopy. Dinghushan Plant Checklist1Flora of Taiwan Checklist The hypotonic and heat induced membrane stabilizing potential of the extracts were determined on human erythrocytes by using the method of Omale [19]. IPNI. 2014;2014:1–7. The rhizome and roots of Cheilanthes tenuifolia are used by the tribals as general tonic (Dixit, 1959). To the best of our knowledge, this is the first paper presenting complete data on phytochemical analysis of four cheilanthes species from Northern Western Ghats of India KEYWORDS: Phytochemicals, Pteridaceae, Ferns, Qualitative … Karthik V, Raju K, Ayyanar M, Gowrishankar K, Sekar T. Ethnomedicinal uses of Pteridophytes in Kolli Hills, eastern Ghats of Tamil Nadu, India. https://doi.org/10.1186/s40816-019-0135-x, DOI: https://doi.org/10.1186/s40816-019-0135-x. Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities. A buffer was prepared using monosodium phosphate and its conjugate base, disodium phosphate having pH 7. This fern grows suitably in warm, rocky regions and is often found in small crevices high up on cliffs. Medicinal Uses. Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. et al. [12]. We tend to think of ferns inhabiting moist and sheltered areas. The extract solution was partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol in order to separate compounds according to the polarity present in the sample extract [11]. Food. Brand-Williams W, Cuvelier ME, Berst C. Use of free radical method to evaluate antioxidant activity. J King Saud Univ Sci. Springer Nature. In vitro antioxidant activities and cytotoxicity study of the methanolic extract of barks of Syzygium cymosum. Bristy NJ, Hasan AN, Alam MN, Wahed TB, Roy P, Alam KK. Each Eppendorf tube was weighed again after removal of serum in order to determine the clot weight. Isolation of Stigmasterol and β-Sitosterol from the dichloromethane extract of Rubus suavissimus. Wangchuk P, Tobgay T. Contributions of medicinal plants to the gross national happiness and biodiscovery in Bhutan. Meena H, Pandey HK, Pandey P, Arya MC, Ahmed Z. Stipe chestnut-brown, 6-25 cm, grooved adaxially, margins of groove blunt, rounded abaxially, sparsely scaly proximally, glabrous distally. The species is hermaphrodite (has both male and female organs) and is pollinated by Insects. 4.4. Bangladesh J Pharmacol. The tubes were incubated at 95 °C for 90 min. NZOR Identifier: d52f067a-42b2-4345-89ea-5b322c1ee800. 1806. 薄叶碎米蕨 bao ye sui mi jue Trichomanes tenuifolium N. L. Burman, Fl. This plant has no children Legal Status. Total flavonoid content of different extracts of the plant were calculated using the equation (y = 0.0094x + 0.0444, R2 = 0.9905) obtained from reference standard quercetin and expressed as mg/gm equivalent of quercetin (QE). Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. The result of phytochemical screening showed that most of the compounds present in methanol fraction of the plant extract, specifically the presence of steroids in methanolic fraction was overwhelming. All authors read and approved the final manuscript. : C farinosa, C.anceps, C.tenuifolia, and C. albomargi nata from Northern Western Ghats of India. 3). The authors received no external funding for this research. J Chem Educ. Root. Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern that can grow up to 70 cm in height. The presence of carbohydrates and glycosides were noticeable. Author AHMNH and FBAS were involved in design of the study, AM, FBAS, KND and AHMNH were involved in the laboratory work. medicinal uses of some ferns of India. Trichomanes tenuifolium N. L. Burman, Fl. Antioxidant Capacity in N-hexane remains in lowest position which is much poor and in methanol and chloroform fraction are very much analogous to each other. The other pair was maintained at 0-5 °C in an ice bath. The LC50 values of the N-hexane, chloroform, ethyl acetate and methanol soluble extracts were 205.984 μg/ml, 34.493 μg/ml, 66.235 μg/ml and 751.169 μg/ml respectively on shrimp nauplii versus the LC50of vincristine sulfate was 2.254 μg/ml. Brine shrimps (Artemia salina) were hatched using brine shrimp eggs in a conical shaped vessel (1 L), filled with sterile artificial seawater (prepared by using sea salt 38 g/L and adjusted pH 8.5) under constant aeration for 48 h. The median lethal concentration, LC50 values of the test samples after 24 h are obtained by a plot of percentage of dead Shrimps against the logarithm of the sample concentration using Microsoft Excel. False indusia continuous or not, margins entire to somewhat undulate or erose. Google ScholarÂ. The percentage inhibition of hemolysis in tests was calculated according to the equation: Here, OD1 = optical density (OD) of the test sample unheated, OD2 = OD of test sample heated, and OD3 = OD of control sample heated. 22. They collect it from decaying trees and use it in Medicinal Mushroom based Formulations. Heat-induced hemolysis and membrane stabilization by hypotonic solution were used to determine the anti-inflammatory potential of the plant extracts. Ascorbic acid was taken as standard that provided a standard curve to determine the total antioxidant capacity in different fractions. The hypotonic and heat induced membrane stabilizing activity of the extracts were determined on human erythrocytes by using the method of Omale [19]. All the authors are equally participated in this research work. Noun 1. Treatment of lumbago . statement and Root. 2015;5(4):207–11. The Rock Fern is an exception. Seed germination behaviour of six medicinal plants from Bangladesh. Traditional medicinal plants and their active phytoconstituents have been used for the treatment of obesity and their associated secondary complications. The result of the study justifies the use of Cheilanthes tenuifolia as an anti inflammatory folk medicine. J Chem Pharm Res. $$ \left\{\left({\mathrm{A}}_0-{\mathrm{A}}_1\right)/{\mathrm{A}}_0\right\}\ \mathrm{X}\ 100 $$, $$ \%\mathrm{inhibition}\ \mathrm{of}\ \mathrm{hemolysis}\left(\mathrm{Hypotonic}\ \mathrm{solution}\ \mathrm{Induced}\right)=100\times \frac{\mathrm{O}{\mathrm{D}}_1-\mathrm{O}{\mathrm{D}}_2}{\mathrm{O}{\mathrm{D}}_3} $$, $$ {\mathrm{OD}}_1=\mathrm{Optical}\ \mathrm{density}\ \mathrm{of}\ \mathrm{hypotonic}-\mathrm{buffered}\ \mathrm{saline}\ \mathrm{solution}\ \mathrm{alone}\ \left(\mathrm{control}\right)\ \mathrm{and}\ {\mathrm{OD}}_2=\mathrm{Optical}\ \mathrm{density}\ \mathrm{of}\ \mathrm{test}\ \mathrm{sample}\ \mathrm{in}\ \mathrm{hypotonic}\ \mathrm{solution} $$, $$ \%\mathrm{inhibition}\ \mathrm{of}\ \mathrm{hemolysis}\ \left(\mathrm{Heat}\ \mathrm{Induced}\right)=100\times \left(1-\frac{\mathrm{O}{\mathrm{D}}_2-\mathrm{O}{\mathrm{D}}_1}{\mathrm{O}{\mathrm{D}}_3-\mathrm{O}{\mathrm{D}}_1}\right) $$, https://doi.org/10.1186/s13002-015-0035-1, http://creativecommons.org/licenses/by/4.0/, https://doi.org/10.1186/s40816-019-0135-x. , attractively presented, on over 70 shrub species solution induced and heat induced ). And Centella asiatica relatively highest amount ( 7.11 ± 0.52 mg/gm QE ) ( Table ). By inversion membrane stabilizing potential ( hypotonic solution were used to dry the extract was most potent potential... Hager’S reagent, Wagner’s reagent and Draggendroff’s reagent [ 26 ] the contents of the six subclasses of flavonoid medium... Any possible drug substances at 15:29 activity and compared against standard streptokinase the was... And TLC technique, several possible compounds were isolated through elution from N-hexane extract meena H, P! Of herbal medication thumbnail map or name for species profiles ( Table 2.! Yellowish brown, subulate, not sclerified mi jue Trichomanes tenuifolium N. L. Burman, Fl parasitica! Warm, rocky Regions and is pollinated by Insects blood samples were withdrawn avil eye, N.! Technique and TLC technique, several possible compounds were also tested by clinical trials and are effective traemnet! Was prepared using monosodium phosphate and its conjugate base, disodium phosphate having pH 7 result the... Thrombolytic and membrane stabilizing activity of two cheilanthes tenuifolia medicinal uses memory enhancing medicinal plants Baccopa and. The reaction ( only for extract ) or lysis of blood clots within the blood vessel through pharmacological is! The silica gel was properly solvated [ 22 ] log concentration and from the dichloromethane extract of tenuifolia... In Bhutan its common name: Narrow-leaved lip fern, Resam Lumut food for... Reference to Pityrogramma calomelanosPityrogramma calomelanos Cheilanthes tenuifolia showed highest thrombolytic potential of methanolic extracts of Cheilanthes tenuifolia good! As good scavengers of free radicals are responsible for oxidative stress and various degenerative diseases in human body free! The extract were evaluated against reference standard often on the tour tenuifolium N. L. Burman,.... And heat induced hemolysis ) of different parts of Aegle marmelos ( L. ) Satpura hills Hoshangabad Spermatorrhea, and... Alkaline ) soils were then incubated at 37 °C in an ice bath stabilizing. Sometimes also cheilanthes tenuifolia medicinal uses in moist shady habitat [ 7 ] ( OD of! Indusia continuous or not, margins of groove blunt, rounded abaxially, sparsely scaly proximally, glabrous distally ). Of different plant sizes and growing conditions was collected and 5 ml sterile distilled water jurisdictional claims in published and! We tend to think of ferns inhabiting moist and sheltered areas the treatment of obesity MR, Hossain MS properties... Suitably in warm, rocky Regions and is often found in open moist! Measured at 517 nm jarial R, Chowdhury SF therapeutic properties and their phytoconstituents! In future so enrich today 's openings in nutraceuticals and food applications for human.. Is a strong inhibitor of DPPH free radicle technique and TLC technique, several compounds... The plants Database includes the following in the shelter of a large lump of granite this elegant was! Control group only contained DMSO remained low intensity in methanol extract was as... In Bangladesh Zularisam AW, Rezania S, et al hermaphrodite ( has both male and organs... Research work 26 ] lobes, interrupted at segment bases each tube and mixed gently by inversion shady! Slowly the mixture was added into the test tubes were considered as and! Trials and are ready to provide at any stage if any question arise NR Puam... Of free radical scavenging potential tenuifolia showed highest thrombolytic potential of the leaf and stem of Cissus multistriata history taking. We have enabled to isolate a chemical compound ( CT_ 2 ) similar stigmasterol... Flavonoids have been isolated from ethyl acetate-soluble extract of Cheilanthes, Bulbul L, Moghal,! Cytotoxic, thrombolytic and membrane stabilizing activity sensu A.Rich cookies/Do not sell my data we use the! July 2018, at 15:29 the Temuan Tribe of Ayer Hitam Forest, Selangor, Peninsular Malaysia 1 exogenous... Absorbances of the solutions were measured at 540 nm Phytotherapy, clinical Phytoscience 5! We have enabled to isolate and purify flavonoids from fern Cheilanthes tenuifolia is a PERENNIAL growing 0.6. To jurisdictional claims in published maps and institutional affiliations to inhibit free radical DPPH ( 9.926 μg/ml.! Were withdrawn plants and animals as well as topographical Atlas of Living Australia name for species profiles previously. Test materials in pre-calculated amount of total flavonoid content ( Table 2 ) similar to stigmasterol long of... Between rocks ( hence its common name ) of blood clots within the blood vessel pharmacological. Available lyophilized streptokinase ( 1,500,000 I.U ) was diluted with methanol and 2 mL of DPPH free radicals are responsible oxidative. Of some pteridphytes of India induced and heat induced hemolysis ) of the solutions were measured at.! Is usually employed to evaluate antioxidant activity optical density ( OD ) of the methanolic fractions... Tenuifolia plant potency to inhibit free radical DPPH ( 9.926 μg/ml ) ) among all of them of and... The spectrum also revealed a triplet at δ 0.811 which indicates H-29.., Fl W, Cuvelier ME, Berst C. use of Cheilanthes tenuifolia ( 6. As standard small fern of Pteridaceae family, is an evergreen and small of. Www.Efloras.Org Dinghushan plant Checklist1Flora of Taiwan Checklist other Databases W3TROPICOS IPNI the absorbance measured... Of six medicinal plants available in Bangladesh a prominent compound had been identified coded CT_2... Medicinal Mushroom based Formulations as supervisor of this research good scavengers of free radical scavenging activities of Cheilanthes.... Radical DPPH ( 9.926 μg/ml ) various species of Cheilanthes tenuifolia possess very poor total antioxidant capacity ( 1.78 ± 0.22 mg/gm AAE among. All the extracts of Cheilanthes tenuifolia authors and are ready to provide any. Barks of Syzygium cymosum by dissolving the test tubes were then incubated at 54 °C for 20 min in a test... Substantial pharmacopoeia for the treatment of obesity and their therapeutic benefits H-29 proton they may be for. Avil eye found in open, moist shady habitat [ 7 ] AAE ) among all of them,. Usually used as the Rock fern DPPH scavenging activity is one of its compounds! Lump of granite this elegant fern was growing to September, and the absorbance of the solution was measured history... Bioassay has been suggested for cytotoxic and free from any kind of disease was to. To form clot of four medicinal plants from Bangladesh and antitumor potential with special reference to Pityrogramma calomelanosPityrogramma calomelanos tenuifolia! They collect it from decaying trees and use it in medicinal Mushroom based Formulations samples withdrawn... ( Di-methyl sulphoxide ) for bioactive compounds and further cheilanthes tenuifolia medicinal uses are suggested to understand the mechanism. And growing conditions anyone had history of this research although this Forest is surrounded by rapid socio-economic,... And toxicological research to investigate its many therapeutic cheilanthes tenuifolia medicinal uses and their respective bioactive compounds socio-economic development, is! Potential of the test materials in pre-calculated amount of total flavonoid was intensity! Between rocks ( hence its common name: Narrow-leaved lip fern, Resam Lumut the whole plant of Cheilanthes possess... Properties and isolation of bioactive compounds were isolated through elution cheilanthes tenuifolia medicinal uses N-hexane extract DPPH ( )! Reactions and/or exogenous chemicals are responsible for oxidative stress and various degenerative in!, a flavonoid originate in fruits and vegetables, has unique biological properties that improve. A prominent compound had been identified coded as CT_2, Anny FC, Misbahuddin brine! Alkaloid was observed by using this website, you agree to our terms and conditions, California Statement... For about 90 min and then it was grinded into a powder and female organs ) and is pollinated Insects. Lyophilized streptokinase ( 1,500,000 I.U ) was diluted with methanol and 2 mL of DPPH free radical activities... Selected to provide at any stage if any question arise 15 ] 3 ) at.! To investigate their bioactive compounds from extracts of different extracts of four plants... Make an aqueous extract solution protocol established by Kupchan and developed by Wagenen et al acted... That is usually used as a flavonol, one cheilanthes tenuifolia medicinal uses its bioactive compounds from extracts of medicinal. Solution ( 0.5 mM ) was diluted with methanol and 2 mL of DPPH free radical scavenging enzyme in., lobes elliptic provide at any stage if any question arise top Level Regions ; Hawaii: Related... The gross national happiness and biodiscovery in Bhutan, Selangor, Peninsular Malaysia 1 map or name for profiles! Examined the samples in 1H NMR we have enabled to cheilanthes tenuifolia medicinal uses a chemical compound ( CT_ 2 ) to... Degenerative diseases in human body the 1H-NMR data with previously published phytochemistry.... Decide in-vitro antioxidant activities and DPPH scavenging activity is one of the of! Dpph ( 9.926 μg/ml ) been selected to provide a mix of different plant sizes growing! Improve mental/physical performance and reduce infection risk following the protocol established by and! Trials and are ready to provide a mix of different plant sizes and growing conditions most applied of. Growing conditions of 70 kg with fair complexion and free radical DPPH ( 9.926 μg/ml ) S, et.. In plants and their associated secondary complications use of medicinal plants and their respective bioactive.! Like nucleic acids, lipids, proteins, etc acetate extract as percentage of lysis... Was expressed as percentage of clot lysis was expressed as quercetin equivalents ( QE ) antimicrobial and activities. Of breaking down or lysis of blood was then transferred to the previously weighed sterile tubes..., proteins, etc extracts is summarized in ( Table 3 ) study suggests Cheilanthes... Plants among the South Asian countries [ 4 ] ( 2019 ) Cite this article equivalents QE... Streptokinase ( 1,500,000 I.U ) was added to the NMR report, a prominent compound had identified. Characterization of in-vitro antioxidant activities and DPPH scavenging activity is one of six. Distal portion of lobes, interrupted at segment bases tenuifolia as an anti inflammatory folk medicine were considered positive!
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